Evaluation of T-cell System

• Quantitative:
  • CBC-look at absolute lymphocyte count
  • Flow cytometry: look at T-cell numbers and subset analysis by looking at CD’s. (eg. mature T cell-CD3, or helper cell CD4, or cytotoxic cell CD8)
• Qualitative:
  • DHT-Delayed Hypersensitivity skin Testing: used if > 1 y/o. Evaluates in vivo T cell function. Eg. Candida, tetanus, Diphtheria, PPD, trichophytan
  • Lymphocyte proliferation assay. Evaluates in vitro T cell fxn. Eg. Tetanus, candida.